FIG. 1.

Abundances of GFP, tk, and gC mRNAs in KOS-, d109-, and d106-infected Vero and HEL cells. Infections, RNA isolation, cDNA preparation, and RT-PCR with primer sets for the indicated mRNAs were performed as described in Materials and Methods. The procedure for creating standard curves for quantification using the indicated primers is also described in Materials and Methods. The graphs indicate the number of RNA molecules of each gene per μg RNA at the indicated time points (2, 4, 8, and 24 h p.i.) in Vero and HEL cells.