FIG. 2.

MN digestion of d109- and d106-infected cells. Nuclei were isolated from d109-, d106-, or mock-infected HEL cells (A) or Vero cells (B) and digested with 20 units MN for 2, 10, or 30 min. The DNA was isolated from the nuclei at 4 and 24 h p.i., purified, and fractionated on 2.0% agarose gels. The agarose gels were stained with EtBr (top) and transferred to Nytran membranes for Southern blot hybridization as described in Materials and Methods. The Southern blots (bottom) were probed with ³²P-labeled HSV BAC DNA, washed, and exposed to X-ray film. Shown are the autoradiographic images of the probed blots (bottom).