FIG. 5.

Binding of H3AcK9 to the tk, gC, and HCMV promoters of d109 and d106 in Vero and HEL cells. ChIP with an antibody to H3AcK9 and RT-PCR were performed as described in the legend to Fig. 3 to determine the percentage of genomes bound by H3AcK9 at 4 and 24 h p.i. in Vero (A) and HEL (B) cells. The error bars represent standard deviations from multiple experiments. In order to compensate for the reduced histone H3 on d106 promoters, AcH3 was normalized to the amount of histone H3 on the genome by dividing the percentage of AcH3 associated with each promoter by the percent histone H3 (Fig. 3) in Vero cells (C) and HEL cells (D).