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. 2009 Jun 24;83(17):8800–8809. doi: 10.1128/JVI.01009-09

FIG. 7.

FIG. 7.

Interactions between segments of the bICP0 E promoter and C/EBP-alpha. (A) Schematic of EMSA probes C1, C2, and C3. C1 contains two putative C/EBP-alpha binding sites found within EP-172. C2 contains the other putative C/EBP-alpha binding site found upstream of the TATA box. C3 contains a putative C/EBP-alpha binding site downstream of the TATA box. The consensus C/EBP binding site in the rat CYPD5 promoter (24) is shown as a comparison to the putative sites in the bICP0 E promoter. (B) EMSA using C1, C2, or C3 probes. Probes were incubated with 30 μg of Neuro-2A cell lysate. Antibodies directed against C/EBP-alpha (A) or C/EBP-beta (B) were used in supershift assays as described in Materials and Methods. (C) To better visualize the supershift for C2 and C3, autoradiography was performed for 24 h versus 4 h as shown in panel B.