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. 2009 Jun 10;83(17):8409–8417. doi: 10.1128/JVI.00796-09

FIG. 6.

FIG. 6.

Sp100B and Sp100HMG influence expression from hdAd vectors. (A and B) HeLa cells were transfected with control siRNA (siCtrl) or pooled siRNA targeting sequences in the mRNAs of all Sp100 isoforms (siSp100). (A) Forty-eight hours later, whole-cell lysates were harvested for immunoblot analysis with anti-Sp100 or anti-α-tubulin. (B) Forty-eight hours after transfection, the cells were infected with hdAd-prok or -euk (MOI, 1 IU/cell). The next day, cell lysates were assayed for β-Gal activity (means ± standard errors; n = 3). The asterisk indicates a significant difference. (C and D) HeLa cells were transfected with pcDNA3 or with plasmids carrying FLAG-tagged Sp100A, Sp100B, or Sp100HMG. (C) Twenty-four hours later, whole-cell lysates were harvested for immunoblot analysis with anti-FLAG and anti-α-tubulin. (D) One day after transfection, the cells were infected with hdAd-prok or -euk (MOI, 1 IU/cell). The next day, cell lysates were assayed for β-Gal activity (means ± standard errors; n = 2).