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The effect of various transcription factor binding sites upstream of two SP1 binding sites on early viral gene expression when one transcription factor binding site is mutated. Transfection of recombinant HCMV Towne BAC DNAs and the measurement of CAT activity or viral DNA synthesis were as described in Materials and Methods. (A) Viral gene expression as measured by CAT activity at various days after transfection. (B) Quantitative PCR of gB DNA at various days after transfection.
