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The CREB transcription factor binding site upstream of two SP1 has the greatest effect on infectious viral replication. Transfection of HCMV Towne BAC DNA and plaque assay in HFF cells were as described in Materials and Methods and elsewhere in the text. (A) Yield of infectious virus at various days after transfection. (B) Comparison of plaque sizes for recombinant viruses dlE-694/-223 and 2SP1 at 21 days posttransfection.
