FIG. 6.

The CREB transcription factor binding site upstream of two SP1 sites has the greatest effect on early viral gene expression in different HCMV permissive cell types. Transfection of wild-type FIX BAC GFP or recombinant FIX BAC DNAs dlE-694/-223, 2SP1 + CREB, 2SP1 + AP1, 2SP1 + 2NF-κB, 2SP + NF1, and 2SP1 into endothelial and epithelial cells, respectively, and viral gene expression as measured by CAT activity were as described in Materials and Methods. (A, B, and C) FIX BAC GFP fluorescence. (D, E, and F) CAT assay of FIX BAC recombinant DNAs in various cell types. (A and D) HAOE cells; (B and E) hepatocytes; (C and F) hTERT-RPE cells.