TABLE 3.

Thermodynamic values associated with the binding of gp120 and NBD-556

gp120 protein	Kd (μM)	ΔG (kcal/mol)	ΔH (kcal/mol)	−TΔSa (kcal/mol)
KB9	1.7	−7.8	−22.6	+14.8
KB9-H66N	NBb	NB	NB	NB
ADA N197S	0.40	−8.7	−12.0	+3.3
ADA N197S-H66N	0.64	−8.4	−13.8	+5.4

^aT, temperature; ΔS, entropy change.

^bNB, no detectable binding.