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. 2009 Jun 10;83(17):8565–8574. doi: 10.1128/JVI.00603-09

FIG. 4.

FIG. 4.

Involvement of mTOR in ST antigen-AMPK-mediated rescue. (a) Immunoblot analysis of whole-cell extracts of glucose-deprived +ST and −ST cells for phospho-p4EBP1 (P-p4EBP1) (Ser65). (b) Immunoblot analysis of whole-cell extracts of rapamycin-treated −ST cells for P-p4EBP1 (Ser65). (c) Photomicrographs represent phase-contrast images of +ST and −ST cells treated with rapamycin. (d) Quantification of trypan blue-positive +ST and −ST cells upon glucose deprivation and rapamycin treatment (n = 3). ***, P < 0.001. (e) Whole-cell extracts of glucose-deprived +ST and −ST cells were subjected to immunoblot analysis for pAMPK (Thr172), total AMPK, pACC (Ser79), total ACC, phospho-raptor (pRaptor) (Ser792), and total raptor. (f) Whole-cell extracts of glucose-deprived +ST and −ST cells were subjected to immunoblot analysis for pAMPK (Thr172), pACC (Ser79), phosphorylated mTOR (pmTOR) (Ser2448), and total mTOR.