TABLE 1.
Peptide-bearing phage eluted from ANDV
| Phage | % Inhibition (SD)a | P valueb |
|---|---|---|
| Phage bearing the following peptides eluted with anti-Gn antibody 6B9/F5 | ||
| Group 1 (<30% inhibition) | ||
| CDQRTTRLC | 8.45 (15.34) | 0.0002 |
| CPHDPNHPC | 9.94 (7.72) | 0.333 |
| CQSQTRNHC | 11.76 (13.25) | 0.0001 |
| CLQDMRQFC | 13.26 (9.92) | 0.0014 |
| CLPTDPIQC | 15.70 (14.05) | 0.0005 |
| CPDHPFLRC | 16.65 (15.22) | 0.8523 |
| CSTRAENQC | 17.56 (16.50) | 0.0004 |
| CPSHLDAFC | 18.98 (20.06) | 0.0017 |
| CKTGHMRIC | 20.84 (7.47) | 0.0563 |
| CVRTPTHHC | 20.89 (27.07) | 0.1483 |
| CSGVINTTC | 21.57 (19.61) | 0.0643 |
| CPLASTRTC | 21.65 (5.98) | 0.004 |
| CSQFPPRLC | 22.19 (8.26) | 0.0004 |
| CLLNKQNAC | 22.34 (7.78) | 0.001 |
| CKFPLNAAC | 22.89 (6.15) | 0.0001 |
| CSLTPHRSC | 23.63 (16.74) | 0.0563 |
| CKPWPMYSC | 23.71 (6.68) | 0.0643 |
| CLQHDALNC | 24.01 (7.60) | 1 |
| CNANKPKMC | 24.67 (11.67) | 0.0004 |
| CPKHVLKVC | 25.30 (28.36) | 0.0003 |
| CTPDKKSFC | 26.91 (11.15) | 0.399 |
| CHGKAALAC | 27.22 (32.53) | 0.005 |
| CNLMGNPHC | 28.08 (21.35) | 0.0011 |
| CLKNWFQPC | 28.64 (18.49) | 0.0016 |
| CKEYGRQMC | 28.76 (29.33) | 0.0362 |
| CQPSDPHLC | 29.44 (31.22) | 0.0183 |
| CSHLPPNRC | 29.70 (17.37) | 0.0061 |
| Group 2 (30-59% inhibition) | ||
| CSPLLRTVC | 33.05 (20.26) | 0.0023 |
| CHKGHTWNC | 34.17 (12.50) | 0.0795 |
| CINASHAHC | 35.62 (13.03) | 0.3193 |
| CWPPSSRTC | 36.75 (26.95) | 0.0006 |
| CPSSPFNHC | 37.78 (7.11) | 0.0001 |
| CEHLSHAAC | 38.47 (7.60) | 0.0115 |
| CQDRKTSQC | 38.74 (9.12) | 0.1802 |
| CTDVYRPTC | 38.90 (25.03) | 0.006 |
| CGEKSAQLC | 39.11 (27.52) | 0.0013 |
| CSAAERLNC | 40.13 (6.33) | 0.0033 |
| CFRTLEHLC | 42.07 (5.01) | 0.0608 |
| CEKLHTASC | 43.60 (27.92) | 0.1684 |
| CSLHSHKGC | 45.11 (49.81) | 0.0864 |
| CNSHSPVHC | 45.40 (28.80) | 0.0115 |
| CMQSAAAHC | 48.88 (44.40) | 0.5794 |
| CPAASHPRC | 51.84 (17.09) | 0.1935 |
| CKSLGSSQC | 53.90 (13.34) | 0.0145 |
| Group 3 (60-79% inhibition) | ||
| CPSNVNNIC | 61.11 (25.41) | 0.1245 |
| Negative control | 0 (6.15) | |
| 6B9/F5 (5 μg/ml) | 26.77 (5.33) | |
| ReoPro (80 μg/ml) | 79.86 (4.88) | |
| Phage bearing the following peptides eluted with anti-Gc antibody 6C5/D12 | ||
| Group 1 (<30% inhibition) | ||
| CHPGSSSRC | 1.01 (7.03) | 0.0557 |
| CSLSPLGRC | 10.56 (13.62) | 0.7895 |
| CTARYTQHC | 12.86 (3.83) | 0.3193 |
| CHGVYALHC | 12.91 (7.32) | 0.0003 |
| CLQHNEREC | 16.79 (13.72) | 0.0958 |
| CHPSTHRYC | 17.23 (14.53) | 0.0011 |
| CPGNWWSTC | 19.34(9.91) | 0.1483 |
| CGMLNWNRC | 19.48 (19.42) | 0.0777 |
| CPHTQFWQC | 20.44 (13.65) | 0.0008 |
| CTPTMHNHC | 20.92 (11.68) | 0.0001 |
| CDQVAGYSC | 21.79 (23.60) | 0.0063 |
| CIPMMTEFC | 24.33 (9.28) | 0.2999 |
| CERPYSRLC | 24.38 (9.09) | 0.0041 |
| CPSLHTREC | 25.06 (22.78) | 0.1202 |
| CSPLQIPYC | 26.30 (34.29) | 0.4673 |
| CTTMTRMTC (×2) | 29.27 (8.65) | 0.0001 |
| Group 2 (30-59% inhibition) | ||
| CNKPFSLPC | 30.09 (5.59) | 0.4384 |
| CHNLESGTC | 31.63 (26.67) | 0.751 |
| CNSVPPYQC | 31.96 (6.51) | 0.0903 |
| CSDSWLPRC | 32.95 (28.54) | 0.259 |
| CSAPFTKSC | 33.40 (10.64) | 0.0052 |
| CEGLPNIDC | 35.63 (19.90) | 0.0853 |
| CTSTHTKTC | 36.28 (13.42) | 0.132 |
| CLSIHSSVC | 36.40 (16.44) | 0.8981 |
| CPWSTQYAC | 36.81 (32.81) | 0.5725 |
| CTGSNLPIC | 36.83 (31.64) | 0.0307 |
| CSLAPANTC | 39.73 (4.03) | 0.1664 |
| CGLKTNPAC | 39.75 (16.98) | 0.2084 |
| CRDTTPWWC | 40.08 (18.52) | 0.0004 |
| CHTNASPHC | 40.26 (4.77) | 0.5904 |
| CTSMAYHHC | 41.89 (8.61) | 0.259 |
| CSLSSPRIC | 42.13 (29.75) | 0.2463 |
| CVSLEHQNC | 45.54 (6.55) | 0.5065 |
| CRVTQTHTC | 46.55 (8.45) | 0.3676 |
| CPTTKSNVC | 49.28 (14.00) | 0.3898 |
| CSPGPHRVC | 49.50 (42.60) | 0.0115 |
| CKSTSNVYC | 51.20 (4.60) | 0.0611 |
| CTVGPTRSC | 57.30 (11.31) | 0.0176 |
| Group 3 (60-79% inhibition) | ||
| CPMSQNPTC | 65.60 (13.49) | 0.014 |
| CPKLHPGGC | 71.88 (27.11) | 0.0059 |
| Negative control | 0.26 (4.53) | |
| 6C5/D12 (5 μg/ml) | 22.62 (8.40) | |
| ReoPro (80 μg/ml) | 80.02 (76.64) |
a
Standard deviations of four experiments are shown in parentheses. Peptide-bearing phage were added at 109 phage/μl.
b
P values for the pairwise amino acid alignment score of each peptide versus that of integrin β3 were determined using an unpaired Student's t test. P values considered statistically significant are shown in bold.