FIG. 5.

Blocking ELISA in the presence of detergent with TBE virus sE dimers and full-length E dimers (solubilized from virions with Triton X-100) and 11 TBE virus-neutralizing MAbs. The domain specificities of the antibodies (DI, DII, and DIII and not assignable [n.a.]) are indicated on the left side, and the designations of the antibodies are indicated at the top of each panel. Serial dilutions of the antigens were preincubated with a predetermined concentration of the respective MAb, and the fraction of MAb not blocked by its reaction with antigen was detected in an ELISA with TBE virus-coated plates (see Materials and Methods). The data shown represent the mean of three independent experiments, and the standard error of the mean is indicated by error bars.