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. 2009 Jun 17;83(17):8525–8535. doi: 10.1128/JVI.00699-09

FIG. 5.

FIG. 5.

Single MA amino acid changes implicated in myristoyl switch regulation improve RRE/Rev-derived Gag assembly efficiency in murine cells. (A) Depiction of N-terminal MA single-amino-acid mutations generated for this analysis. Basic residues are indicated by “+.” (B) 3T3 cells were transfected with the indicated MA mutant and cultured in the presence of saquinavir, and the cells and supernatants were processed for quantitative assembly assay as described for Fig. 2C. Release factors were normalized to the wild-type (WT)-RRE plus Rev sample. The change in the release factor is indicated above each bar. (C) A twofold 4×CTE plasmid dilution series comparing the assembly efficiency of Gag (L21S) mutants derived from 4×CTE-dependent transcripts (lanes 1 to 5) to that derived from RRE/Rev-dependent transcripts (lane 6). As for Fig. 2A, VLP production was assessed both by immunoblotting and p24Gag ELISA. The change in p24Gag release is indicated above each bar.