FIG. 2.
Generation of a stable cell line, BSR-VP6, expressing VP6 constitutively. (A) Schematic representation of the expression vector, pCAG-PBTV10VP6. VP6 protein was expressed under the control of the CAG promoter, whereas a puromycin resistance gene (Puror) was expressed under the control of the simian virus 40 early promoter. (B) Immunoblot of clone 22-2 with BSR-VP6 (lane 1) and BSR (lane 2) cells. The blot was probed using a rabbit polyclonal antiserum raised against BTV-10 VP6. (C) Immunofluorescence of BSR-VP6 cells (left) and BSR cells (right). Detection of VP6 was performed using a guinea pig polyclonal antiserum raised to BTV-10 VP6, followed by Alexa Fluor 488 goat anti-guinea pig IgG.