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Anterograde spread in Campenot chambers. A total of 1 × 105 PFU WT, mutant, or rescue strain HSV-1 was added to the S chamber. The titers of the contents of the S (A and B) and N (C and D) chambers were determined on Vero cells 24 (A and C) or 48 (B and D) hpi. Results shown are means ± standard errors of six chambers per virus strain at each time point. *, P < 0.05; **, P < 0.01; ***, P < 0.001.
