FIG. 2.

Humoral immune reaction against MV and HBsAg in MV-susceptible mice. Groups of 12 or 13 animals were inoculated by the intraperitoneal route with 5 × 10⁴ TCID₅₀ of MVvac2 (Vac), MVvac2(HBsAg)N (N), and MVvac2(HBsAg)P (P). Serum samples obtained at 28 days postimmunization were assayed for MV neutralization (A) (reciprocals of neutralizing titer) and anti-HBs levels (B) (quantitative ELISA, Bioelisa anti-HBs; Biokit, Barcelona, Spain). Each dot represents one animal; the mean is indicated by a horizontal bar. All experimental procedures were performed as sanctioned by the Mayo Clinic Institutional Animal Care and Use Committee.