TABLE 1.
Genetic alterations found in T7 suppressor phagesa
Trial | Suppressor no. | Genetic alteration (nt)b | Outcome in gene expression |
---|---|---|---|
A | 1 | T10422C | gp3 F56L |
B | 2 | ||
3 | |||
C | 4 | A10482G | gp3 K76E |
5 | A10575G | gp3 T107A | |
T10666C | gp3 V137A | ||
6 | Δ10132 | Frameshift in gene 2.8 | |
7 | Δ10132 | Frameshift in gene 2.8 | |
8 | Δ11195-11196 | Deletion in noncoding region between genes 3.5 and 3.8 | |
9 | Δ11195-11196 | Deletion in noncoding region between genes 3.5 and 3.8 | |
10 | Δ11195-11196 | Deletion in noncoding region between genes 3.5 and 3.8 |
A total 10 suppressor phages were isolated from three independent trials, and their DNA sequences in class II genes (gene 1.4 to gene 6.3) were analyzed. Genetic changes found from the phages and their consequences in gene expression are indicated.
nt, nucleotide.