TABLE 4.
Ability of gene 3 mutations to suppress T7 DNA polymerases of low processivity
| Phage | Complementation efficiency with the indicated mutant protein(s) [no. of PFU with mutated protein(s)/no. of PFU with wt protein(s)]a
|
|
|---|---|---|
| gp5-4N/gp5 wt | [gp5(T327C)/Trx(C35S)]/(gp5 wt/Trx wt) | |
| T7Δ5 | 0.08 | 0.006 |
| Suppressor 1 | 0.6 | 0.5 |
| Suppressor 4 | 0.9 | 0.2 |
| Suppressor 5 | 0.6 | 0.5 |
a
Recombinant proteins were expressed from plasmids under the control of a T7 promoter in either E. coli strain C600 (for single gene 5 expression) or E. coli A307 (for both gene 5 and Trx expression). After infection with the indicated T7 phage, the number of plaques was counted, and PFU were determined. Mutated residues are in parentheses. Data were obtained from at least two experiments. wt, wild-type protein.