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. 2009 Jun 24;83(17):8492–8501. doi: 10.1128/JVI.00922-09

FIG. 4.

FIG. 4.

Interaction of LANA with oriLyt. (A) Schematic of HHV8 oriLyt region containing C/EBPα transcription factor binding sites and location of flanking PCR amplification primers. RRE, Rta-responsive element. (B) LANA interacts with oriLyt. ChIP assay from HHV8 BAC36-infected cells using LANA-specific antibodies and primers flanking the C/EBPα binding motif sites within oriLyt. (C) The interaction of K-bZIP with oriLyt is dependent upon LANA expression. ChIP assays were performed using cells infected with the recombinant HHV8 bacmids that are defective for the expression of LANA or K-bZIP. The left and bottom panels show ChIP assays from LANA-knockout bacmid-infected cells. The right panel shows a ChIP assay from ΔK-bZIP bacmid-infected cells. ChIP assays were performed using anti-UL84-, anti-LANA-, anti-K-Rta-, or anti-K-bZIP-specific antibodies.