FIG. 1.

Gag_241-249-specific CD8⁺ T-cell induction by prophylactic vaccination. (A) Schema of the cDNA construct encoding the Gag_236-250-EGFP fusion protein. A DNA fragment that encodes a 31-mer peptide including the Gag_236-250 sequence was introduced into the 5′ end of the EGFP cDNA. (B) Gag_241-249-specific CD8⁺ T-cell frequencies 1 (open boxes) and 2 weeks (closed boxes) after F(−)SeV-Gag_236-250-EGFP boosting in group III macaques. (C) SeV-EGFP-specific CD4⁺ (open boxes) or CD8⁺ (closed boxes) T-cell frequencies 2 weeks after F(−)SeV-Gag_236-250-EGFP boosting in group III macaques. p-b, postboost.