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Accumulation of phosphorylated eIF2α (Phospho-eIF2α) is associated with loss of HSV-2 vhs nuclease activity. Wild-type 129Sv/Ev MEFs were infected at an MOI of 10 with the indicated viruses. Lysates were collected at 6 h p.i. and analyzed by Western blot analysis for phosphorylated eIF2α (middle band indicated by arrows) (A), total eIF2α (B), or HSV-1 and HSV-2 proteins (C).
