FIG. 3.

Replication of an HSV-2 vhs mutant virus is restored to wild-type levels in PKR^−/− and S51A MEFs. (A) Wild-type 129Sv/Ev MEFs were infected at a low MOI (0.01) with the indicated viruses. Samples were collected 24 h p.i. Data represent virus titers from duplicate samples from one experiment and are consistent with published data. Wild-type B6, PERK^−/−, and GCN2^−/− MEFs (B); 129Sv/Ev and PKR^−/− MEFs (C); or B6 and S51A MEFs (D) were infected at a low MOI (0.01). Viral titers from samples collected 24 h p.i. are represented as the means ± standard error of the mean of the results for two experiments (B) or three experiments (C and D). *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, P > 0.05.