FIG. 3.

GFP-UL44 associates with dsDNA and the nuclear matrix, in contrast to GFP-UL44L86A/L87A and GFP-UL44Δloop. The intracellular localization of the indicated GFP fusion proteins was investigated 24 h after transfection of Daoy cells, prior to treatment (NT), or after treatment with 1% NP-40 for 15 min at room temperature (NP-40) or after incubation with 40 U of DNase I/ml for 1 h at room temperature, followed by incubation with NaCl 2 M for 5 min (DNase I, NaCl). The bright-field (BF) and the green channel (GFP) are shown, with cellular dsDNA being shown in blue (Hoechst).