TABLE 1.
Plasmids used in this study
| Plasmid | Characteristic(s) | Reference |
|---|---|---|
| pcDNA3.1(−) | Eukaryotic expression vector carrying a CMV promoter and BGH polyadenylation signal | Invitrogen |
| pIRES-EGFP | Source of the EGFP gene | Clontech |
| pYS1190 | Source of the mCherry gene | Unpublished |
| pTM-PolI-WSN-All | Eight-unit plasmid for transcribing PB2, PB1, PA, NP, HA, NA, M, and NS vRNAs by means of HPI | 16 |
| pCAWS-NP | Eukaryotic expression of NP used as the helper plasmid | 16 |
| pYA3994 | Source of prokaryotic GFP cassette | Lab collection |
| pYA4464 | Vector with p15A ori sequence and Cmr cassette | Lab collection |
| pYA4749 | GFP expression vector with p15A ori constructed by fusing DNA segments from pYA3994 and pYA4464 | This study |
| pYA4337 | PB2 gene inserted into pcDNA3.1(−) | This study |
| pYA4338 | PB1 gene inserted into pcDNA3.1(−) | This study |
| pYA4339 | PA gene inserted into pcDNA3.1(−) | This study |
| pYA4379 | CPI and MTI cloned into pcDNA3.1(−) to create a bidirectional vector to synthesize vRNA by means of CPI and mRNA by means of the CMV promoter | This study |
| pYA4383 | PB2 cDNA cloned into pYA4379 to synthesize mRNA by means of the CMV promoter and vRNA by means of CPI | This study |
| pYA4384 | PB1 cDNA cloned into pYA4379 to synthesize mRNA by means of the CMV promoter and vRNA by means of CPI | This study |
| pYA4385 | PA cDNA cloned into pYA4379 to synthesize mRNA by means of the CMV promoter and vRNA by means of CPI | This study |
| pYA4386 | NP cDNA cloned into pYA4379 to synthesize mRNA by means of the CMV promoter and vRNA by means of CPI | This study |
| pYA4387 | EGFP gene cloned into pYA4379 to synthesize mRNA by means of the CMV promoter and antisense RNA (vRNA-like) by means of CPI | This study |
| pYA4380 | CPI and MTI cloned into modified pcDNA3.1(−) to synthesize vRNA | This study |
| pYA4388 | HA cDNA inserted into the AarI sites in pYA4380 to synthesize vRNA by means of CPI | This study |
| pYA4389 | NA cDNA cloned into pYA4380 to synthesize vRNA by means of CPI | This study |
| pYA4390 | M cDNA cloned into pYA4380 to synthesize vRNA by means of CPI | This study |
| pYA4391 | NS cDNA cloned into pYA4380 to synthesize vRNA by means of CPI | This study |
| pYA4392 | EGFP gene cloned into pYA4380 to transcribe antisense RNA (vRNA-like) by means of CPI | This study |
| pYA4688 | CPI replaced with HPI in pYA4392 to transcribe the EGFP gene into antisense RNA (vRNA-like) | This study |
| pYA4519 | Eight influenza cDNA cassettes cloned into one plasmid to synthesize vRNAs by means of CPI and PB2, PB1, PA, and NP mRNA by means of the CMV promoter | This study |
| pYA4731 | mCherry gene cloned between the CMV promoters and BGH terminator in pcDNA3.1(−) | This study |
| pYA4732 | CMV-mCherry-BGH cassette from pYA4731 inserted into pYA4519 at the SrfI site | This study |