FIG. 8.

Complementation of vA17Li infectivity by A17 cleavage site mutants. Cells were infected with 5 PFU of vA17Li in the presence of IPTG (+IPTG) or absence of IPTG (all other lanes) and either not transfected (first lane) or transfected with a plasmid expressing GFP, full-length (wild type) A17 (A17wt), or mutated A17 in which glycine 16 or 185 was replaced by alanine regulated by a synthetic early/late promoter. The cells were harvested after 24 h, and virus titers were determined by plaque assay in the presence of 100 μM IPTG. Error bars show standard deviations.