TABLE 1.
Oligonucleotides used in this study
| No. | Name | Sequence (5′-3′)a |
|---|---|---|
| 1 | PV-3B-3C-Glu-Gly-for | CGG ACA GCA AAG GTA GAA GGA CCA GGG TTC G |
| 2 | PV-3B-3C-Glu-Gly-rev | CGA ACC CTG GTC CTT CTA CCT TTG CTG TCC G |
| 3 | 3B-3C-QGtoGG-f | CGG ACA GCA AAG GTA GGA GGA CCA GGG TTC GAT TAC |
| 4 | 3B-3C-QGtoGG-r | GTA ATC GAA CCC TGG TCC TCC TAC CTT TGC TGT CCG |
| 5 | pRLuc-BglII-r | CGC AGA TCT CCA CTT CTT TGC CA |
| 6 | pMoRA-SnaBI-f | TTA TGT ACG TAC CAC CAGGAGCTC CAG TGC CCG AG |
| 7 | pRLucRA-3B-Y3F-f | GGA CAC CAG GGA GCA TTC ACT GGT TTA CC |
| 8 | pRLucRA-3B-Y3F-r | GGT AAA CCA GTG AAT GCT CCC TGG TGT CC |
| 9 | pLP-Rev-TRE BamHI 3CD for | GCG GGA TCC GCC ACC ATG GGA CCA GGG TTC GAT TAC |
| 10 | pLp-RevTRe-3CD-SphI-rev | ACG CGC ATG CTT ACT AAA ATG AGT C |
| 11 | 3A-SacII-f | GCG GAA TTC CCG CGG TGG AGG ACC ACT CCA GTA T |
| 12 | 3D seq 1085 Rev | CTC CCA TGT GAC TGT TTC AAA TG |
| 13 | 3C 1 seq | GGA CCA GGG TTC GAT TAC G |
| 14 | 3D seq 138 Rev | GTC TGT CTT AAG CCT GGG ATC G |
| 15 | 3D seq 100 | GTT TGA AGG GGT GAA GGA A |
a
Restriction sites are shown in boldface; codons containing nucleotide changes are italicized.