FIG. 3.
muSOX is necessary for viral replication and host shutoff during infection. (A) EcoRI and PvuII digests of WT MHV68, MHV68ΔmuSOX, and MHV68ΔmuSOX-MR. In the PvuII digest, asterisks represent cleavage products generated as a result of introducing an additional PvuII site upon deletion of the muSOX gene. (B) Growth curve comparing viral titer following transfection of 3T3 cells with WT, MHV68ΔmuSOX, and MHV68ΔmuSOX-MR BAC DNA. Limit of detection is 10 PFU/ml. Data are a compilation of three independent replicates with the WT and deletion BACs and two independent replicates with the MR BAC. (C) The indicated BAC DNAs were transfected into BHK cells in the presence of 200 μg/ml PAA. BAC-derived GFP mRNA abundance was determined by qPCR at different time points posttreatment with 5 μg/ml ActD. Errors bars show the standard error between samples from three independent experiments. t1/2, half-life.