FIG. 4.

SOX homologs of gamma-HVs localize to both the nucleus and cytoplasm. (A) 293T cells were transfected with empty vector or HA-tagged SOX (KSHV), muSOX (MHV68), BGLF5 (EBV), and AE (HSV-1). Twenty-four hours posttransfection, cells were subjected to IFA with anti-HA antibodies. (B) HFF cells were either mock infected or infected with KSHV and lytically reactivated with an adenoviral vector expressing RTA. At the indicated times, samples were subjected to IFA with anti-ORF45 (KSHV lytic marker) (center panels) and anti-SOX (bottom panels) antibodies. (C) 3T3 cells were either mock infected or infected with BAC-derived MHV68. At the indicated times, samples were subjected to IFA with anti-muSOX antibodies. GFP is encoded by the BAC and serves as a marker of infection. Bottom panels show a magnified view of representative muSOX-expressing cells at each time point. All samples were costained with DAPI to visualize nuclei. α, anti.