TABLE 1.
Primers used for plasmid construction
| Construct | Primer name | Primer sequence |
|---|---|---|
| ΔmuSOX BAC | ORF36-DsRed | GGAAGGGTCGATTATTCTGGATTTTTTTGAAGCCTCCTCCGAGAACGTCATC |
| ΔmuSOX BAC | DsRed-ORF37 | CGCCACCACCTGTTCCTGCCAGATAGTATTGTGTATGAAATAAAGTCCAGATATAAG |
| ΔmuSOX, ΔmuSOX MR BACs | BamHI-ORF36-fwd | ATTAGGATCCGACAGCACTGTACAAGGAGGC |
| ΔmuSOX, ΔmuSOX MR BACs | ORF38-NotI-rev | TTAAGCGGCCGCGTGTTTGCCTCGTTCATATTCAATG |
| HA-NRS-muSOX | KpnI-HA-NRS-fwd | GGGGTACCATGGCTTACCCATACGATGTACCTGACTATGCGCCAAAAGTGAACCGAGGAAAA |
| HA-NRS-muSOX | EcoRI-NRS-rev | CGGAATTCTCCACTCTTAGAATTGAAGCC |
| HA-ΔNRS-muSOX | KpnI- HA-ΔNRS-fwd | GGGGTACCATGGCTTACCCATACGATGTACCTGACTATGCGAAACGATCTGCAGCGGAG |
| HA-ΔNRS-muSOX | EcoRI- HA-ΔNRS-rev | CGGAATTCTTTCGAGGGCACTACAGCCCG |
| GFP-29aa (for ΔNRS) | EcoRI-GFP-29aa-fwd | GGGAATTCCAGGATGATGGCACGCTGCCC |
| GFP-29aa (for ΔNRS) | EcoRI-GFP-29aa-rev | CGGAATTCATTGATCCTAGCAGAAGCACA |
| HA-UL12 | EcoRI-UL12-fwd | GGAATTCATGGCTTACCCATACGATGTACCTGACTATGCGATGGAGTCCACGGTAGGC |
| HA-UL12 | NotI-UL12-rev | ATAGTTTAGCGGCCGCTCAGCGAGACGACCTCCC |
| HA-UL12-NLSmut | UL12-NLSmut-fwd | CGTGGCCCCGACAGCCCCCCCGCGGCCCCCCGCCCTAACAGTCTTCCG |
| HA-UL12-NLSmut | UL12-NLSmut-rev | CGGAAGACTGTTAGGGCGGGGGGCCGCGGGGGGGCTGTCGGGGCCACG |
| HA-muSOX | EcoRI-HA-ORF37 fwd | CGGAATTCATGGCTTACCCATACGATGTACCTGACTATGCGATGGAAGGGTCGATTATTC |
| HA-muSOX | NotI-ORF37 rev | ATAGTTTAGCGGCCGCTTAGGGGGTTATGGGTTTTCT |