FIG. 1.

Production of IFN-α, IFN-β, TNF-α, and IL-12/23p40 by BMDC in response to iPPVO. (A) Flt3L-generated BMDC were stimulated with iPPVO (MOI = 5), CpG-ODN (1 μM), and LPS (5 μg/ml). After 48 h of incubation, the concentrations of IFN-α, IFN-β, TNF-α, and IL-12/23p40 in the culture supernatants were measured by sandwich ELISA. Each column represents the mean ± the standard error of the mean (SEM) of at least duplicate cultures. The results of one representative experiment of at least three independent experiments are shown. The levels of LPS-induced IFN-β were below detection in this experiment. However, small amounts of LPS-induced IFN-β could be observed in single experiments (data not shown). (B) Supernatants of iPPVO-stimulated Flt3L-generated BMDC were harvested at the indicated time points, and the amounts of IFN-α and IL-12/23p40 were determined. The results of one of two independent experiments are shown (duplicate cultures).