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TLR2 and TLR4 are not involved in iPPVO-triggered TNF-α and IL-12/23p40 release. Wild-type (WT), TLR2−/−, and TLR4−/− BMDC generated in the presence of Flt3L were stimulated with iPPVO at an MOI of 5 and with the indicated control stimuli for 48 h. Subsequently, supernatants were harvested and levels of TNF-α and IL-12/23p40 determined by sandwich ELISA. Pooled data of two independent experiments are shown. ***, P < 0.001; **, P < 0.01.
