FIG. 3.

Subcellular localization of OSBP and OSBP mutants. Huh7 cells were transfected with vectors encoding the FLAG-tagged wild-type (WT) and indicated mutant OSBP genes. Cells were analyzed by confocal immunofluorescence microscopy with anti-FLAG and anti-TGN46 antibodies (see Materials and Methods). Panels in the left column show FLAG-tagged OSBP (red). Panels in the center column show subcellular localization of TGN46 as a marker of the TGN. Panels in the right column show superimposed images of OSBP and TGN46.