FIG. 2.

MHV-1 replication is controlled by both innate and adaptive immune responses. Virus titers were measured by plaque assays in the lungs, livers, spleens, and brains of wild-type (WT) B6 mice, anti-NK1.1-treated B6 mice, B6-Rag1-KO mice, and B6-IFN-αβR-KO mice 3 days (D3) and 10 days (D10) after intranasal infection with MHV-1 (10⁵ PFU/mouse). All B6-IFN-αβR-KO mice succumbed to infection prior to day 10. Five mice per group were analyzed at each time point, and data are represented as the number of PFU/g of tissue from individual mice. Statistically significant differences in titers between wild-type B6 mice and the rest of the experimental groups were determined using an unpaired Student's t test and are indicated by an asterisk. The limit of detection is indicated in each graph by the horizontal line parallel to the x axis; mouse groups are indicated on the x axes of panels D and H, and values in graphs above are aligned similarly.