TABLE 1.
Infectivity for genomic components of CLCuRaV and CbLCuV in the presence of betasatellites to N. benthamiana
| Inoculum | No. of plants infected/No. of plants inoculated |
Symptom severityb | Betasatellite replication (no. of plants showing betasatellite replication) | ||
|---|---|---|---|---|---|
| Expt 1 | Expt 2 | Expt 3 | |||
| CLCuRaV | 0/9 | 0/9 | 0/9 | 0 | |
| CLCuRaV + CLCuMuBMul | 9/9 | 9/9 | 9/9 | 5 | Yes (27) |
| CLCuRaV + CLCuMuBrMul/Raj | 9/9 | 9/9 | 9/9 | 4 | Yes (27) |
| CLCuRaV + CLCuMuBBur | 9/9 | 9/9 | 9/9 | 5 | Yes (27) |
| CLCuRaV + ChLCB | 9/9 | 9/9 | 9/9 | 4 | Yes (27) |
| CLCuRaV + CLCuMuBBurΔ995-1095 | 9/9 | 6/9 | 9/12 | 4 | Yes (24) |
| CLCuRaV + CLCuMuBBurΔ150-840 | 0/9 | 0/9 | 0/12 | 0 | Yes (30)a |
| CLCuRaV + CLCuMuBBurΔ1130-116 | 0/9 | 0/9 | 0/12 | 0 | No |
| CbLCuVA | 0/9 | 0/9 | 0/9 | 0 | |
| CbLCuVA+B | 7/9 | 9/9 | 9/9 | 3 | |
| CbLCuVA + CLCuMuBMul | 0/9 | 0/9 | 0/9 | 0 | No |
| CbLCuVA + CLCuMuBrMul/Raj | 0/9 | 0/9 | 0/9 | 0 | No |
| CbLCuVA+B + CLCuMuBMul | 6(2)/9c | 7(2)/9c | 7(1)/9c | 4(5)c | Yes (25) |
| CbLCuVA+B + ChLCB | 7/9 | 9/9 | 9/9 | 3 | No |
| CbLCuVA+B + CLCuMuBrMul/Raj | 8/9 | 9/9 | 9/9 | 3 | No |
| CbLCuVA+B + CLCuMuBBur | 7/9 | 8/9 | 9/9 | 4 | Yes (24) |
| CbLCuVA+B +CLCuMuBBurΔ995-1095 | 7/9 | 9/9 | 8/9 | 4 | Yes (24) |
| CbLCuVA+B +CLCuMuBBurΔ150-840 | 7/9 | 8/9 | 9/9 | 3 | Yes (24) |
| CbLCuVA+B +CLCuMuBBurΔ1130-116 | 7/9 | 9/9 | 9/9 | 3 | No |
| CbLCuVA+B + CLCuMuBMul/Mut | 9/9 | 8/9 | 9/9 | 5 | Yes (26) |
a
Replication of CLCuMuBBurΔ150-840 was assessed only in the infiltrated leaves.
b
Symptom severity is indicated on a scale of 0 to 5, with 5 indicating severe symptoms associated with infection of N. benthamiana with CLCuRaV and CLCuMuBMul (see Materials and Methods).
c
For the CbLCuVA+B with CLCuMuBMul inoculations, plants exhibiting two distinct symptom severity levels were noted. The figures in parentheses are the numbers of plants showing the more severe symptoms (severity level of 5).