FIG. 2.

Kinase-deficient tel1 alleles partially inhibit de novo telomere elongation. (A) Southern blotting analysis for the de novo telomere elongation assay in strains with TEL1 and tel1 alleles. Strains used were yYM159 (tel1Δ) and spores derived from yYM278-282 (2FLAG-TEL1 and 2FLAG-tel1s). The time, in hours, shows the duration for which the telomere seed was allowed to be extended. Plasmid pRS416 in the strains was used as a control to another experiment and did not affect this assay (data not shown). (B) Quantitative analysis of results shown in panel A. The fraction of the extended telomere seed is plotted against the time of telomere seed elongation. Data from three independent experiments are presented as means ± standard errors of the means. Solid lines, wild-type (2FLAG-TEL1) and tel1Δ strains; dashed lines, strains with point mutations.