FIG. 4.

XRS2 C-terminal mutants are partially defective in the de novo telomere elongation assay. (A) Southern blotting analysis for the de novo telomere elongation assay in strains with 13myc-tagged XRS2 and xrs2 alleles (derived from yYM286, -288, -289, -292, and -293). The time, in hours, shows the duration for which the telomere seed was allowed to be extended. (B) Quantitative data analysis of the results shown in panel A. The fraction of the extended telomere seed is plotted against the time of telomere seed elongation. Data from two independent experiments are presented as means ± standard errors of the means. Solid line, the wild-type strain (XRS2-13myc); lines with short or long dashes, the mutant strains.