FIG. 5.

tel1^KD combined with xrs2 C-terminal mutants synergistically inhibits de novo telomere elongation. (A) Southern blotting analysis for de novo telomere elongation in strains with different combinations of 2FLAG-TEL1 and XRS2-13myc alleles (derived from yYM319, -321, -300, -301, -308, -305, and -324). The time, in hours, shows the duration for which the telomere seed was allowed to be extended. (B) Quantitative data analysis of the results shown in panel A. The fraction of the extended telomere seed is plotted against the time of telomere seed elongation. Data from two independent experiments are presented as means ± standard errors of the means (data for strains 2FLAG-TEL1 XRS2-13myc and 2FLAG-tel1-D2612A/N2617A XRS2-13myc were from one experiment, but similar results have been obtained in other experiments). Solid line, the wild-type strain; lines with long dashes, strains with single mutations; lines with short dashes, the tel1Δ strain and strains with double mutations.