FIG. 6.
The combination of tel1KD and an xrs2 C-terminal mutant resembles tel1Δ at native telomeres, and overexpressing tel1KD does not rescue the short telomeres. Southern blotting analysis results show the native telomere lengths of strains expressing combinations of the TEL1 and XRS2 alleles. The wild-type (WT) strains were derived from yYM256. Strains for lanes 3 to 20 were derived from yYM309, -310, -268, -269, -274, -275, -311, -312, -325, -326, -333 to -336, and -327 to -330. Both TEL1 and XRS2 alleles are tagged (except for tel1Δ and xrs2Δ). The blot was probed with a poly(dA-dC)·(dG-dT) probe. Sizes of DNA markers (in kb) are indicated on the left.