FIG. 2.

Knockdown of menin interferes with differentiation of murine 3T3-L1 cells. (A) 3T3-L1 cells were transfected with the indicated siRNA oligonucleotides. After 6 days of differentiation, oil red O staining was performed to assess lipid accumulation. (B) Immunoblot (IB) analysis of global menin, PPARγ (the Cell Signaling Technology antibody recognizes multiple forms of PPARγ), and FABP4 levels in undifferentiated (Und.) cells and siRNA-treated cells was performed. α-Tubulin was used as a loading control. The positions of molecular mass markers, with their respective molecular masses in kilodaltons, are indicated next to the blots. (C) RT-PCR analysis of total mRNA levels of Men1, Pparg, and Fabp4 in siRNA-treated 3T3-L1 cells. The values shown represent averages and standard deviations. mRNA levels were normalized against β-actin mRNA.