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. 2009 Jul 13;29(18):5060–5069. doi: 10.1128/MCB.01001-08

FIG. 3.

FIG. 3.

Menin is required for PPARγ-dependent adipocyte differentiation. (A) Polyclonal MEN1−/− and wild-type MEF cells that ectopically express PPARγ were treated with 1 μM rosiglitazone for 10 days. Lipid vesicle formation was assessed by oil red O staining (40× magnification, inset represents 10× magnification). (B) Menin and menin L22R were reexpressed in MEN1−/− MEFs expressing PPARγ. The presence of menin and PPARγ (SC antibody recognizes PPARγ2 specifically) was visualized by immunoblotting (IB) using α-tubulin as a loading control in total cell lysates of two monoclonal cell lines carrying the empty vector, two cell lines reexpressing wild-type menin, and two cell lines expressing menin L22R that had been treated with 1 μM rosiglitazone for 14 days. The positions of molecular mass markers, with their respective molecular masses in kilodaltons, are indicated next to the blots. (C) Oil red O staining of the cell lines described in panel B.