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. 2009 Jul 6;29(18):5115–5127. doi: 10.1128/MCB.01969-08

FIG. 6.

FIG. 6.

Acetylated NPM1 occupies the TNF-α gene promoter. (A) A ChIP assay was done with anti-NPM1, anti-AcNPM1-01, and anti-RNAP II antibodies along with an immunoglobulin G (IgG) control in KB cells at the TNF-α promoter. Values are relative to the immunoprecipitated input DNA. (B) A ChIP assay was performed following 48 h of either control siRNA or NPM1 siRNA no. 2 transfection of KB cells with anti-RNAP II and anti-acetylated histone H3 antibodies. Results and error bars are averages and standard deviations of PCRs from three independent ChIP experiments. The data were analyzed by paired t test (*, P < 0.05). (C) A reChIP assay was done by carrying out first immunoprecipitation with either anti-NPM1 or anti-AcNPM1-01 antibody and then anti-RNAP II antibody at the TNF-α promoter. A reChIP assay was also done with anti-NPM1 antibody, followed by anti-AcNPM1 antibodies. Crude chromatin was used as the input for reChIP assays. Results and error bars are averages and standard deviations of at least three independent PCRs from two independent reChIP experiments.