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. 2009 Jul 13;29(18):5094–5103. doi: 10.1128/MCB.00299-09

FIG. 3.

FIG. 3.

Brd4 knockdown leads to abnormal chromosome segregation in HFKs. (A) Primary HFKs were transfected with either Brd4 siRNA Si1 or a control siRNA. Cells were fixed and subjected to anti-Brd4 immunofluorescent staining and DAPI nuclear staining. Arrows mark the enlarged Brd4 knockdown cells with binucleated or polynucleated structure. (B) Cells with abnormal nuclei were quantitated from 100 cells. Three independent experiments were performed. The averages ± standard deviations are shown.