FIG. 7.

Brd4 regulates Aurora B activation during mitosis. (A) T98G cells were cultured in DMEM and 10% FCS. At 60 to 80% confluence, the cells were incubated with starvation medium (DMEM with 4.5 g/liter glucose) for 72 to 96 h. Cells were then released into release medium (DMEM with 4.5 g/liter glucose and 10% FCS). At 31 h after release, the peak for mitotic cells (>50%), protein lysates were prepared and analyzed by Western blotting using anti-Aurora B antibodies. Actin is shown as a loading control. (B) T98G cells were transfected with either Brd4 Si1 (KD) or the control siRNA (CO). At 24 h posttransfection, cells were synchronized as described above. At the peak of mitosis (31 h after release from starvation medium), protein lysates were prepared and analyzed by anti-Aurora B Western blotting. Actin is shown as a loading control.