FIG. 1.

Rep2 is an unstable protein whose levels oscillate along with cell cycle. (A) Rep2 is an unstable protein. Western blot analysis using anti-HA antibodies shows decreased levels of Rep2-HA in cells at various time points after treatment with CHX. The S-phase cyclin Cig2 is a known unstable protein that served as a positive control. PSTAIR (Cdc2 conserved sequences, regardless of phosphorylation status at the residue 15 tyrosine) was used as loading control. (B to D) Cdc10, Res1, and Res2 are stable proteins. (E) Levels of Rep2 oscillate with the cell cycle. The level of Rep2 in synchronous cdc25-22 culture was monitored at various time points after release to the permissive temperature (@24°C). Peaks of mitotic indexes (M) and septation indexes (S) are indicated. The arrows indicate the peaks of the oscillated Rep2 or Cig2 levels.