FIG. 8.

Disruption of ste9 alleviates the cds1Δ defect in the transcriptional response to HU. (A) Induction of the MBF-dependent genes upon HU treatment in ste9Δ, cds1Δ ste9Δ, and cds1Δ cells. The display of the transcription levels is identical to that in Fig. 5A. (B) Average expression profiles of the MBF-dependent genes in response to HU treatment. (C) Disruption of ste9 diminishes the pattern of oscillation of Rep2 levels during the cell cycle. Rep2 levels in cdc22-25 ste9Δ synchronous cultures were determined at various time points after release to the permissive temperature (@24°C). The peak of the mitotic index (M) is indicated. Arrows indicate the peak of the oscillated Cig2 levels.