Skip to main content
. Author manuscript; available in PMC: 2010 Jun 15.
Published in final edited form as: Cancer Res. 2009 Jun 2;69(12):5115–5125. doi: 10.1158/0008-5472.CAN-09-0645

Fig.1. Analysis of clonal ovarian cancer cultures that were either resistant or susceptible to lysis by Ad5/35.IR-E1A/TRAIL.

Fig.1

A) Cytolysis caused by Ad5/35.IR-E1A/TRAIL in clonal cultures derived from biopsy ovc316. Clonal cultures indicated in black were subjected to DNA expression array analysis. Standard deviation was less than 10% of the average for all samples. Infection at MOIs 10 and 200 pfu/cell resulted in a similar distribution. B) Focal adhesion, tight junction (TJ) and adherens junction (AJ) pathways. Genes that were found upregulated in arrays are marked red. Down-regulated genes are marked green. TJ proteins, include claudins and occludin. AJ proteins include E-cadherin. The cytoplasmic domain of E-cadherin interacts with ß-catenin and p120-catenin. Claudins and occludin interact with ZO-1, and subsequently with F-actin via cingulin. Caldesmon inhibits Arp2/3-mediated actin polymerization. Phosphoinositide 3-kinase (PI3K) and Focal adhesion kinase (FAK) are involved in the regulation of Rho GTPases downstream of integrin signaling. Rho kinase (ROCK) is activated by RhoA. Vinculin and α-actin crosslink the cytoskeleton to focal adhesion spots. Vimentin is the major intermediate filament (IF) protein of mesenchymal cells that is involved in regulation of attachment, migration, and cell signaling. Palladin functions as a scaffold that regulates actin organization. Profilin is involved in turnover of the actin filament network. C) Flow cytometry analysis of resistant and susceptible clones. Two representative clones are shown. D) Immunofluorescence analysis of epithelial marker proteins (upper panels) and mesenchymal marker proteins (lower panels) on resistant and susceptible clones. Representative clones are shown.