Fig.4. Analysis of ovc316 xenografts.
A) Analysis of tumor sections. Upper panel: Expression of E-cadherin (green) and laminin (red) Middle panel: Co-localization of CD46 (green) or αV integrins (green) with the tight junction protein claudin 7 (red). Lower panel: Viral hexon and E-cadherin expression at day 8 post injection of 2×109 pfu of Ad5/35.IR-E1A/TRAIL; In vivo GFP expression after intratumoral injection of Ad5/35.GFP. In vivo βGal expression after intravenous injection of Ad5/35. βGal. B) Flow cytometry of cell suspensions and cultured ovc316 cells at passage 1 and 20. C) Western blot for key members of pathways that regulate tight junction reorganization and EMT. D) Effect of inhibitors {exoenzyme C3 from Clostridium botulinum (inhibitor of Rho A, B and C GTPases), H-1152 (Rho-kinase inhibitor), Clostridium difficile toxin B (inhibitor for Rho, Rac, and Cdc42), Wortmannin (inhibitor of PI3K)} on viability of Ad5/35.IR-E1A/TRAIL infected R/E and S/M cells. Cell viability was measured at day 4 after infection at an MOI of 100 pfu/cell. Significance was compared to infected, Mock treated cells. ***p<0.001, **p<0.01, *p<0.05