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. Author manuscript; available in PMC: 2010 Jun 15.
Published in final edited form as: Cancer Res. 2009 Jun 2;69(12):5115–5125. doi: 10.1158/0008-5472.CAN-09-0645

Fig.6. Analysis of infectivity of ovarian cancer cells by different Ad serotypes.

Fig.6

A) Immunofluorescence analyses on R/E cells. Left upper panel: Expression of HSPGs (green) and claudin 7 (red) on R/E cells. Left lower panel: Cy3-Ad3 and Cy3-Ad35 attachment in R/E cells (right panel). Right panel: Effect of Ad 3, 5, 7, 11, 14, and 35 infection (MOI 100pfu/cell) on cell morphology and E-cadherin (green) expression in R/E cells. Viral replication was visualized by staining for hexon (red). The analysis was done at day 4 p.i. B) Flow cytometry analyses of Ad infected cells (same conditions as in B). C and D) In vivo transduction of wAd3 and wtAd35. A total of 2×109 pfu of Ad3 and Ad35 was intratumorally injected into subcutaneous ovc316 tumors. C) Transduction was quantified by qRT-PCR for hexon mRNA using pan-serotype hexon primers that can detect both Ad3 and Ad35 hexon mRNA (23). N=5. D) Ovc316 tumor volume after mock injection or intratumoral injection of 2×109 pfu of wtAd3 or wtAd35, N=5.