Skip to main content
. Author manuscript; available in PMC: 2010 May 1.
Published in final edited form as: Science. 2009 Mar 19;324(5927):651–654. doi: 10.1126/science.1171641

Fig. 2.

Fig. 2

NAMPT, NAD+, and SIRT1 regulate CLOCK:BMAL1 activity. (A-B) NAD+ levels (A) and relative expression levels of Pepck, G6Pase, and Igf-1 RNA (B) in primary hepatocytes incubated with 200nM FK866 for 24 hours (n=3/group). (Pepck, phosphoenolpyruvate carboxykinase; G6Pase, glucose-6-phosphatase; Igf-1, insulin-like growth factor-1). (C-E) Relative luciferase activity of Per2-luciferase in the presence of: (C) 10nM FK866 for 24 or 48 hours, (D) increasing doses of Sirt1 (200, 400, 800 ng), and (E) Sirt1 (800 ng) alone or Sirt1 (800 ng) and 10nM FK866 for 48 hours. *p<0.05; **p<0.01; ***p<0.001; NS not significant. All data is presented as mean ± SEM.