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. 2002 Dec;8(12):1379–1384. doi: 10.3201/eid0812.020224

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Mitochondria transmembrane potential and caspase activities measurement. HeLa-CD4 cells were transfected with pcWNV-Cp-DJY (a) or pcDNA3.1 (b), and their mitochondria transmembrane potential was measured after 48 h by ultraviolete illumination. A colorimetric caspase activity assay was performed with pcWNV-Cp-DJY– or pcDNA3.1-transfected cells for caspase-3 (c) or caspase-9 (d) activity. As a specificity control, the inhibitor LEHD-FMK for caspase-9 was added to the reactions along with relevant substrate (d). A specific positive control was used for assay validation (magnification: 1000X [a and b]).